A Highly Specific Protease - Based Approach for 1 the Detection of Porphyromonas gingivalis in the 2 Diagnosis of Periodontitis

نویسندگان

  • Wendy E. Kaman
  • Fabiano Galassi
  • Johannes J. de Soet
  • Sergio Bizzarro
  • Enno C.I. Veerman
  • Alex van Belkum
  • John P. Hays
  • Floris J. Bikker
چکیده

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Rezaei F1, Chalabi M1, Moghim SH2, Moghareh Abed A3, Faghri J1 1. Instructor, Department of Microbiology, Faculty of Medicine, Lorestan University of Medical Sciences 2. MSc in Microbiology, Medical Biology Recearch Center, Kermanshah 3. Assistant Professor, Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences 4. Assistant Professor, Department of Periodontolo...

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Highly specific protease-based approach for detection of porphyromonas gingivalis in diagnosis of periodontitis.

Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides that contain d-amino acids, led to the discovery of five P. gingivalis-specific ...

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The Relationship between Porphyromonas gingivalis and Rheumatoid Arthritis: a Cross-Sectional Clinical, Microbiological, and Molecular Approach

Background: The relationship between rheumatoid arthritis and poor oral hygiene has longbeen proven. Colonization of Porphyromonas gingivalis in the oral cavity can play an important role in rheumatoid arthritis. The aim of this study was to determine whether there is a relationship between rheumatoid arthritis disease and the presence of P. gingivalis. <stro...

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بررسی اثر آنتی‌باکتریال اسانس دارچین (Cinnamon) بر رشد Porphyromonas gingivalis حاصل از پاکت‌های عمیق بیماران مبتلا به پریودنتایتیس مزمن (in vitro)

  Background and Aims : Antibiotics are commonly used for controlling the growth of porphyromons gingivalis (P.g) which is one of the most important etiologic factors in the periodontal diseases. Different side effects of synthetics and chemical drugs such as increasing the drug resistancy in the human pathogens have led to study on the herbal antibacterial effect. The aim of this study was to ...

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Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples.

Porphyromonas gingivalis is a major pathogen in destructive periodontal disease in humans. Detection and quantification of this microorganism are relevant for diagnosis and treatment planning. The prevalence and quantity of P. gingivalis in subgingival plaque samples of periodontitis patients were determined by anaerobic culture and real-time PCR amplification of the 16S small-subunit rRNA gene...

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تاریخ انتشار 2011